Coding

Part:BBa_K5300009:Design

Designed by: Dini Piao   Group: iGEM24_CAU-China   (2024-09-21)


pfa3 coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2776
    Illegal NheI site found at 6940
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2148
    Illegal BglII site found at 4680
    Illegal BglII site found at 6996
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 516
    Illegal NgoMIV site found at 3012
    Illegal NgoMIV site found at 4371
    Illegal NgoMIV site found at 6811
    Illegal NgoMIV site found at 6849
    Illegal AgeI site found at 3048
    Illegal AgeI site found at 3861
    Illegal AgeI site found at 3882
    Illegal AgeI site found at 4308
    Illegal AgeI site found at 4651
    Illegal AgeI site found at 6070
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2877
    Illegal SapI site found at 4848
    Illegal SapI site found at 6048
    Illegal SapI site found at 7603
    Illegal SapI.rc site found at 172
    Illegal SapI.rc site found at 1077


Design Notes

Referencing the codon preference and GC content of rhizobia, perform codon optimization on gene clusters


Source

Aetherobacter fasciculatus (SBSr002)

References